Brain Stroke is one of the prominent causes of death without an efficacious treatment. Fragaria nubicola have potential antioxidant activity due to the presence of phenolic compounds and may be proved as cytoprotective against ischemia-reperfusion induced brain injury. We studied the effect of fresh fruit juice of Fragaria nubicola (10 ml/kg, p.o.) and vitamin E as reference standard drug on 30 min induced ischemia, followed by reperfusion, with the help of evaluation parameters such as neurobehavioral tests consisting of neurodeficit score, beam walk test, rota rod test, hanging wire test and elevated plus maze. The biochemical parameters measured in animals’ brain were nitric oxide, malondialdehyde, superoxide dismutase and catalase, in control and treated rats. The fresh fruit juice of Fragaria nubicola treated groups showed a statistically significant improvement in the neurobehavioral parameters such as motor performance [neurological status, significant increase in grasping ability (P<0.05), forelimb strength (P<0.05) improvement in balance and co-ordination (P<0.05)]. The biochemical parameters in the brains of rats showed a significant enhanced activity of enzymatic antioxidants such as catalase (P<0.01), superoxide dismutase (P<0.01), significant reduction in the total nitrite (P<0.01) and lipid peroxidation (P<0.01). The above observations suggest that juice of F. nubicola fruits (strawberry) have shown the most pronounced cytoprotective activity.