Acyclovir was formulated as oral controlled release matrix tablets using natural and synthetic polymers separately or in combinations. Tablets were prepared by direct compression method. The tablets were evaluated to thickness, weight variation test, drug content, hardness, friability and in vitro release studies. All the formulations showed compliance with pharmacopoeial standards. The results of dissolution studies indicated that formulation F3 was most successful of the study. The formulation F3 exhibited Anomalous (non-Fickian) diffusion mechanism. Based on the results of in-vitro studies it was concluded that the natural and synthetic polymers can be used as an efficient matrix former to provide controlled release of acyclovir. FT-IR study indicate the absence of interaction between acyclovir and excipients.