The high distribution of resistance of a wide range of microorganisms to the diverse range of antimicrobial agents is attracting a great deal of attention. Development of effective and safe drugs based on nano principles are now extensively utilized to fight against pathogenic microorganisms. Among the various route, infection through cross contamination on various objects including wound dressing causes severe health risks in hospitalized patients. In the present study, chitosan polymer stabilized silver nanoparticles incorporated levofloxacin nano drug conjugate was coated on wound dressing by pad dry and the effect of coating on mechanical properties such as tensile strength, bursting strength, air permeability and anti-bacterial activity against human pathogenic bacteria has been carried out. Surface topography of coated wound dressing was characterized by scanning electron microscopy (SEM) which revealed the complete distribution of nano conjugate on the fiber surface with the size range of 70-85nm.Further characterization by Fourier transform infrared spectroscopy (FTIR) showed characteristic modification in the absorbed peaks. Distinct changes in physical properties was not recorded except air permeability. Nano formulation coating showed increased air permeability. Anti-bacterial activity of the nano formulated drug coated wound dressing showed distinct pattern of inhibition.