Fatty acid content of the fresh water fish Anabus testudineous (Local Name: Kawoi) were examined by TLC and GCMS. It has been confirmed that the fish lipid contains approximately 20.72% saturated fatty acid(SFA), 41.98% monounsaturated fatty acid(MUFA) and 1% polyunsaturated fatty acid(PUFA). The components of SFA are Palmitic acid C16:0(19%), Tetradecanoic acid C14:0(1.8%) and Stearic acid C18:0(0.76%). The components of MUFA are Oleic acid C18:1(40.4%) and Eicosaenoic acid C20:1(1.5%). The fish contains 1% of Arachidonic acid C20:4 which is an important natural product.