The consumption of Garlic as spice for food and medicinal purposes is very common among Northern Nigerians. It is believed that Garlic helps fight infections thereby keeping the body healthy. This study was designed to investigate the effect of aqueous extract of Garlic (Alllium sativum) on in vivo leukocytes mobilization in Albino strain Wistar rats. Twenty (20) Albino Wistar rats, divided into four groups of five animals each were used for this study. The group 1 (control group) received 1ml of distilled water, groups 2 and 3 received graded doses of 50 and 100 mg/kg aqueous extract of Garlic, respectively, while the fourth group received Diclofenac (50mg/kg). After one hour, the animals all received 1ml of 3% (w/v) agar suspension in normal saline intra-peritoneally. The animals were killed after four hours (4hrs). The total and differential leukocytes counts were then carried out on the peritoneal fluid obtained from them. The total leukocytes count in the experimental groups were found to be higher than the control group, but the increase was statistically insignificant (p>0.05). The differential leukocyte counts in the control groups also showed a non significant increase in lymphocyte and neutrophils counts when compared with the control groups. The findings in this study showed that aqueous extract of Garlic has no significant effect on leukocytes mobilization in albino strain Wistar rats.