The microbiota has increasingly gained attention because of its relationship linked to the development of human diseases. Periodontal disease is one of the disorders induced by microorganisms, which cause bad breath, swollen and bleeding gums, plaque and even tooth loss. Here, we established an anaerobic MALDI Biotyper system for in vitro diagnosis of oral microbiota to monitor the distribution of bacteria in oral cavity of human. The Bruker database used in this study contains 5,989 species of bacteria were applied to MALDI-TOF to detect oral microbiota.
