The paper-based colorimetric assays have been widely reported in literature being cost-effective, not requiring additional components (i.e. pump) for microfluidic handling of the solution, and avoiding the sample treatment thanks to the filtering property of the paper. In the last decade, the electroanalysis has discovered the utility of using paper as electrode-active support, converging the reported advantages of paper with the features of electroanalysis such the high sensitivity, selectivity, and the capability to work in complex matrices (e.g. coloured samples). Herein, we described the research activity carried out in the last 5 years at Nanobiosensing Lab and SENSE4MED at the Department of Chemical Science and Technologies of Tor Vergata University aimed to develop sustainable and cost-effective (bio)sensors for application in environmental, agrifood, security, and biomedical fields.