Medicinal plants contain biologically-active compounds which have the capacity to prevent, manage or cure various human and animal diseases. The critical point of decision on the possible potency and efficacy of a drug that is being developed depends on the bioactive components of the source plant. This study was designed to evaluate the phytochemistry and mineral element composition of G. barbadense. The phytochemical analysis of methanolic extract of Gossypium barbadense revealed the presence of alkaloid (3.82±0.17), flavonoid (2.80±0.18), total phenols (5.94±0.41), cyanogenic glycosides (18.07±0.54) and saponins (7.28±0.19) in mg/100g. Anthraquinones and terpenoids were absent. The mineral element examination showed that iron (544.81 ± 0.0024), magnesium (447.05 ± 0.0051), zinc (13.56 ± 0.0011), calcium (17.76 ± 0.001) and potassium (3.321 ± 0.0027) were present.