Human cytomegalovirus (HCMV) is a major public health problem throughout the world. In HIV infected persons, HCMV is considered an AIDS-defining infection, indicating that the T-cell count has dropped to low levels. This work was aimed at determining the seroprevalence of CMV and HIV in antenatal patients attending Primary Health Centers in parts of Kaduna State, Nigeria. Serological screening for CMV IgG antibodies was done using Enzyme Linked ImmunoSorbent Assay (ELISA) to detect IgG and HIV screening using Determine HIV1/2 kits. Three hundred and sixty samples were collected, out of which three hundred and fifty seven (99.2%) were positive for IgG and three (0.8%) for HIV. Co-infection of CMV (IgG) and HIV occurred in 0.8% of the total population and was found in age groups less than 30 years. Prevalence of CMV IgG increased with age with 100% prevalence in age groups of greater than 29 years and HIV in age groups less than 20 and 20-29. It is concluded that there was a high seroprevalence of CMV IgG among pregnant women investigated which means CMV is common in the study area and most women have been exposed to the virus and protective antibodies developed. Low HIV prevalence could be due to the effectiveness of HIV awareness programs which are beginning to yield result. Precautions should therefore be taken to avoid reinfection of this virus especially in the HIV positive patients which is a threat to the unborn child with a significant risk of mother soma to child transmission. It is hereby advocated that relevant vaccines should be made available to protect women of child bearing age and pregnant women.