Hyaluronic Acid (HA) is an important macromolecular polymer widely existing in the skin, which can lock water molecules in the skin. Due to the barrier effect of skin stratum corneum, high molecular weight HA cannot enter the deep skin, while low molecular weight HA has the ability limited to maintain water. Nowadays, HA injection is widely used as a supplement method, which brings many disadvantages due to its invasiveness. Therefore, in this study, the ionic liquid induction technology was used to transform the macromolecular HA into the supermolecular state. Through in vitro transdermal experiment and human skin moisturizing experiment, it was proved that the super molecular state HA has better transdermal and skin moisturizing ability, which provide a new potential method for non-invasive exogenous supplementation of HA.