A simple, isocratic rapid stability-indicating ultra-performance liquid chromatography (UPLC) method was developed with 5 minutes run time and validated for the simultaneous quantitative determination of hydrochlorothiazide, ramipril and telmisartan present in tablets. Chromatographic separation achieved isocratically on Waters Acquity BEH Shield RP18 column (100 mm x 2.1, 1.7μm) column. The separation was achieved on simple Isocratic method. The mobile phase consisted of a mixture of 0.1% triethylamine at pH 3.5 with methanol and acetonitrile (3:2:5 v/v) and the flow rate was 0.15 mL/min. The column temperature was maintained at ambient and the detection was carried out at 215 nm. The retention times of Hydrochlorothiazide, Ramipril and Telmisartan are 1.5, 1.9 and 2.9 minutes respectively. The method was validated in terms of system suitability, linearity, precision, limit of detection, limit of quantitation and accuracy. The developed method was linear for hydrochlorothiazide, ramipril, telmisartan in the range of 6.2-18.7 μg/mL, 1.2-3.7 μg/mL and 20-60 μg/mL respectively. The accuracy of the method was evaluated in the range of 80% to 120% in triplicate and the mean recoveries obtained for hydrochlorothiazide, ramipril, telmisartan were 99.7%, 99.6% and 100.3% respectively. Validation parameters such as specificity and robustness were also determined. The method was found to be rapid and stability-indicating, which can be applied for simultaneous quantitative determination of hydrochlorothiazide, ramipril and telmisartan present in combination tablets.
