A simple, reliable and stability indicating reversed-phase high-performance liquid chromatographic method has been developed and validated for the simultaneous determination of citicoline and piracetam in their synthetic mixtures and combined tablet formulations. Both drugs were separated on a Chromatopak C18 250 mm × 4.6mm Column packed with 10μm particles. The optimized mobile phase was a 90:10 (v/v/v) mixture of 10 mM potassium phosphate buffer (pH 3.5), pumped at a flow rate of 0.8 ml/min. UV detection was performed at 215 nm. The method was validated in the concentration ranges of 17.5-32.5μg/ml for citicoline and 28-52μg/ml for piracetam, where it demonstrated good linearity with r2= 0.999 and 0.998 (n = 3), respectively. The method demonstrated to be robust, resisting to small deliberate changes in pH, flow rate and composition (buffer: organic ratio) of the mobile phase. The applicability of the method was demonstrated by determining the drug content of commercial pharmaceutical formulations, where it exhibited good performance.