The exorbitant costs of culture media have deprived the use of readymade culture media such as Potato Dextrose Agar (PDA) in schools and laboratories with financial limitations. Generally legume seeds are found to be a good protein source for nutritional purposes. This study was carried out to find the feasibility of using legume seeds as an alternative nutrient source to grow fungi. Cowpea, green gram, black gram and soya meat (processed soya bean) were used in this study. The test organisms used were Aspergillus, Trichoderma, Fusarium, Sclerotium and Pencillium sp. On average Fusarium sp shows significantly (p<0.05) higher growth (5.85±0.18) in blackgram and Aspergillus shows significantly (p<0.05) less growth (1.58±0.31) in PDA. In comparison with the performance on conventional potato dextrose agar (PDA) media, the prepared protein formulations were found to be relatively cheap and good alternative culture media for mycological studies.