This work was conducted to evaluate the effect of ethanolic extract of Caralluma diazielli on liver enzymes in fructose-induced diabetes in Wistar rats. Induction of diabetes was done by dissolving 20g/100ml (20%) of fructose in distilled water. After which the animals were randomly assigned into 5 groups of 5 rats each. Group 1 negative control received distilled water( 5mg/kg), Group 2 received positive control received glibenclimide orally (1mg/kg b w), Group 3 received 200mg/kg b w of extract of C.diazielli orally, Group 4 received 100mg/kg b w of extract of C.diazielli orally Group 5 received 50mg/kg b w of extract of C.diazielli orally. The results showed There was a significant decrease (p<0.05) levels of all serum liver enzymes (AST, ALT and ALP) in all groups administered to the extract of C.diazielli when compared to the negative control group. The preliminary phytochemical screening of C.diazielli revealed the presences of cardenolides carbohydrate flavonoid and glycosides. Also the Acute toxicity of the extract was found to be 2.154 mg/kg orally.