Background: Cyclophosphamide is used as anticancer therapy and previous studies have been reported its hepatotoxic effect.
Purpose: This study was performed to inspect the antioxidant ameliorative influence of metformin as co-therapy on the hepatotoxicity caused by cyclophosphamide in the treatment of different malignancies.
Methods: Male Sprague Dawley rats were divided into four groups; Control group (C), Metformin (MET), Cyclophosphamide (CP), Protector group (CP.MET). At day 14 all rats were weighed and euthanized. For estimation of both hepatic structure and function as well as the oxidative stress, hepatic tissue and peripheral blood samples were collected.
Results: The results showed that CP produces many structural and functional damage of the liver. While, pretreatment of the rat with Metformin showed significant decrease in serum liver enzyme activity, significant enhancement of oxidative stress. Also, there are enhancements in liver tissue structure.
Conclusion: Depending on the previous results we recommend the routine usage of metformin with CP in the treatment of different diseases.