Background: According to the International Diabetes Federation (IDF) and the World Health Organization (WHO) data there are 382 million people living with diabetes in the world in 2013. 2035 this number will increase to 592 million people. One of the natural resources that has the potential as anti-diabetic but has not been used optimally is Gelidium sp. which contains polysaccharides and polyphenols which can play a role in increasing HDL (High Density Lipoprotein) levels. Purpose: The purpose of this study was to determine the polysaccharide activity against the reduction in blood of male mice (c L). The sample used is Gelidium sp. extracted to obtain polysaccharide compounds and polyphenols which will test anti-hyperglycemia activity and in 4 treatment groups namely positive control group using Akarbose, negative control given 1% NaCMC and each treatment given dose variation of Agarose Dosage I 1.25 mg/20 grams BB Mice and Agarose Dosage II 2.5 mg/20 grams BB Mice. Result: The results of the two-way ANOVA statistical test on fasting blood glucose levels between the negative control group to positive control and agarose dose of 2.5 mg/kg BW mice showed a significant difference with a value of P <0.05. Whereas between negative groups with other treatment groups P value> 0.05 means that there is no significant difference.