Origanum vulgar is a perennial herb belongs to Origanum. The aim of this study was to overview its anti-inflammatory, antioxidant, anticancer and anti-microbial effects. This review article was carried out by searching studies in PubMed, Medline, Web of Science, and IranMedex databases. The initial search strategy identified about 103 references. The search terms were “Origanum vulgare”, “Anti-inflammatory”, “Antioxidant, anticancer, antimicrobial effect”, “pharmacological effects". It is commonly used for treatment of inflammatory, different kinds of cancer including Colon, lung cancers due to its most component 4-terpineol and microbial diseases. It was said to possess antioxidant effect including iron chelating, scavenging activity, and the redox state cells modification because of presence of phenolic compound including ferulic, rosmarinic, p-coumaric and caffeic, while predominant flavonoids were quercetin, apigenin kaempferol. Origanum vulgar is used for the treatment of various diseases such as different kinds of cancers and possess lots of effects as antibacterial, antioxidant, anti-inflammatory and anticancer effects. In this study, Anti-inflammatory, Antioxidant, anticancer, antimicrobial effect of this plant are presented using published articles in scientific sites. Besides, it was said to be good for cancer treatment.