In the present study an attempt was made to evaluate the antimicrobial potential of culture filtrate of Streptomyces sp. VITBT7 and biologically synthesized silver nanoparticles using the culture filtrate of VITBT7. Actinomycetes isolates obtained from soil samples were screened for antimicrobial activity against selected fungal and bacterial pathogens by well diffusion method. The potential isolate was identified by molecular taxonomic characterization. The culture filtrate of the potential isolate was assessed for the synthesis of silver nanoparticles. The synthesized silver nanoparticles were characterized for surface plasma resonance (SPR) peak, shape and size distribution by TEM analysis. Out of 240 actinomycetes colonies recovered, 19 isolates showed mild to moderate antimicrobial activity and one potential isolate showed broad spectrum of activity against tested microbial pathogens with higher zone of inhibition against Pseudomonas aeruginosa and Aspergillus niger. The isolate was identified to be belonged to the genus Streptomyces and designated as Streptomyces sp. VITBT7 (accession number JX188053). The culture filtrate synthesized silver naoparticles (AgNPs) within 24 h. The biologically synthesized AgNPs showed SPR peak at 420 nm and were found to be spherical in shape with the size range of 20-70 nm. Synthesized AgNPs also exhibited antimicrobial activity against fungal and bacterial pathogens. Synthesized AgNPs by Streptomyces sp. VITBT7 could be used effectively to control fungal and bacterial pathogens. The secondary metabolites produced by Streptomyces sp. VITBT7 could be responsible for observed antimicrobial activity.