The main objective of the present study is to evaluate the antimicrobial and antioxidant potential of Dhasalavana dhravagam (DLD) a well-known classical drug selected from the Siddha classical literature, “Kannusamiyam Ennum Vaidhya Segaram. The antimicrobial property of the DLD was studied against Gram-negative and Grampositive microorganisms using the agar well diffusion method. The result of antimicrobial screening study projects that the drug DLD exhibited bigger zone of inhibition of about 08- 26 mm against Staphylococcus aureus followed by this 09- 25 mm of zone against Klebsiella pneumonia, 09- 24 mm of zone against Bacillus Subtilis and 07- 22 mm zone against Salmonella typhimurium at the concentration of 250mg/ml. Antioxidant potential of DLD was evaluated by using DPPH radical scavenging assay. The result obtained from the study shows that DLD possess potential antioxidant activity (91.3%) relatively near to the standard drug. From the study it was concluded that the drug DLD has promising antimicrobial, antioxidant activity and hence this siddha formulation may serve as a prospective drug for treating several infectious diseases in near future.