Antibiotic resistance is growing and has limited ability of physicians to treat some infectious diseases. Discovery of new antibiotics or semi-synthetic derivatives affecting on resistant infections are main objectives for treatment of infectious diseases. The aim of this study was to investigate antimicrobial activity of Hydro-alcoholic extracts of ginger (Zingiber officinale) and Mallow (Malva sylvestris) against Pseudomonas aeroginosa, Staphylococcus aureus, Meticillin resistant Staphylococcus aureus and Listeria monocytogenesis as a number of important factors and infectious microorganisms. Antimicrobial effects of Hydro-alcoholic extracts of ginger (Zingiber officinale) and Mallow (Malva sylvestris) was evaluated by micro-plate dilution and disk diffusion methods. MIC of ginger extract for Staphylococcus aureus, Meticillin resistant Staphylococcus aureus, Pseudomonas aeroginosa and Listeria monocytogenesis were 52, 52, 416 1nd 52 μg/ml and MBC of this extract were 104, 104, 416 and 104 μg/ml, respectively. Growth inhibition zone of ginger extract for these bacteria were 16, 9, 7 and 8 mm, respectively. Mallow extract had no antibacterial effects on studied bacteria. Obtained results showed ginger extract can be introduced as bioactive antibiotic in traditional medicine, microbiology and pharmacology sciences.