Tropane alkaloid, scopolamine is medicinally important compound produced by many plants of Solanaceous species. The present study was to investigate the antioxidant and antiglycation effects of this compound in culture of rat liver tissue. In this study, scopolamine at different concentrations were titred on rat liver cells. Then, the activity of glutathione peroxidase, superoxide dismutase and catalase as well as glyoxal and 2,2-diphenyl -1-picrylhydrazyl inhibition were measured by spectophotometry and changes in malondialdehyde (MDA) in liver cells were measured by high-performance liquid chromatography. The antioxidant and antiglycation activities of scopolamine increased as its concentrations increased in the liver cells, representing promotion of reactive oxygen species generation compared to control. Scopolamine exerts antioxidant and antiglycation activities in rat liver cells.