Pattavia and Nanglae are famous pineapple species (Ananas comosus L. Merr) from Northern Thailand. The objective of this study was carried out antiproliferative activity, phytochemical content and antioxidant of both ethanolic Pattavia and Nanglae extracts. The results showed that Pattavia and Nanglae extracts were effective to growth inhibiton of Hep G2 cells lines with IC50 at 22.40 and 24.28 μg/ml, respectively. Total phenolic contents in Pattavia and Nanglae were 8.20 ± 0.10 and 9.66 ± 0.18 mg gallic acid equivalent/g extract. Total flavonoid in in Pattavia and Nanglae were 1.17 ± 0.08 and 1.15 ± 0.02 mg quercetin equivalent/g extract, respectively. The extract of Pattavia showed scavenge hydrogen peroxide with IC50 value at 106.75 μg/ ml better than Nanglae with IC50 value at 109.10 μg/ ml. Pattavia and Nanglae extracts could change Fe3+ to Fe2+ by using ferric reducing antioxidant power (FRAP) with value of 33.74 ± 0.14 and 40.50 ± 0.64 mM/ g extract, respectively. Both the ethanolic extracts of Pattavia and Nanglae had antioxidant properties. Phenolic and flavonoid compounds might have the important roles in antiproliferative and antioxidant activities of the pineapple extracts.