The effect of salinity stress on some agronomical and physiological traits was evaluated for 272 F7 recombinant inbred lines (RILs) derived from a cross between Roshan and Sabalan wheat varieties. Experiment was conducted using a randomized completely block design with three replications. Genotypes were grown in tap water (EC=0.5dsm-1) and saline water (EC=18dsm-1) as control and salt stress treatment with hydroponics in greenhouse. Saline-related traits including sodium and potassium concentrations in shoot and roots, chlorophyll content, plant height, dry and fresh weight of shoot were measured at seedling stage. Significant differences were observed between salinity treatments for all measured traits, except for the chlorophyll content. Differences among RILs were significant for all traits. Salinity stress decreased K+ concentration and K+/Na+; however Na+ concentration was increased in Roshan and Sabalan genotypes and all RILs under saline conditions. Also, there was a lower transfer of Na+ from root to the shoot and higher ability of leaves for exclusion Na+ in the salt tolerant genotypes. Roshan had high amounts of K+ and K+/Na+ in shoot under stress treatment. Moghan3 (as sensitive control variety) showed the most reduction in shoot dry matter under saline conditions, so it can be considered as the most sensitive genotype. One sensitive and tolerant line founded among 272 RILs compared with parental and control varieties. Tolerant line (line 90) had less Na+ and more K+, resulting in higher K+/Na+ in shoot, and produced more dry matter compared with tolerant Roshan and Arg (as tolerant control variety) varieties. In contrast, the sensitive line (line 33) had higher Na+ and less K+/Na+, dry matter compared with sensitive Sabalan and Moghan3 varieties.