Blood stream infection is an important cause of morbidity and mortality. Etiology of this infection also varies with the geographical location, antibiotic practices. Aim of the present study is to identify the type of organisms isolated from blood culture as well as to determine their antimicrobial susceptibility pattern. During January 2011 to December 2011, a total of 479 blood samples were cultured in the Department of Microbiology, IMS & Sum Hospital. Positive blood cultures were subjected to appropriate identification methods. Antimicrobial susceptibility testing was then undertaken according to CLSI guidelines. Positive growth was seen in 126 (26.3 %) samples. Most common organism isolated was Staphylococcus aureus (92 cases, 73 %) followed by Acinetobacter (14 cases, 11%). Of the staphylococcus isolates 26% were resistant to Oxacilin. Thus it is concluded that there is a rising incidence of Staphylococcal as well as Acinetobacter septicemia replacing the common pathogens like E.coli, Klebsiella and Pseudomonas. The cause and risk factor for this alarming trend has to be looked into.