Acinetobacter baumannii is the most common and increasingly important nosocomial pathogen associated with VAP. The aim of our study was to compare the capacity and performance of sheep blood agar, MacConkey agar and CHROMagar Acinetobacter for isolation and identification of A baumannii in tracheal aspirates specimens obtained from patients with Ventilator-associated pneumonia. (VAP) .Specimens was plated onto three media including: 5% sheep blood agar (SBA), MacConkey agar, and CHROMagar Acinetobacter. SBA was the gold standard to which all media was compared. There were 100 specimens collected during the study period from August 2012 through November 2012. SBA and CHROMagar detected 39 of 39 (100%) Acinetobacter isolates. CHROMagar Acinerobacter had 100% sensitivity and specificity. Nearly all isolates of Acinetobacter baumannii were multiple Drug resistant (MDR). Colisitin was the sole effective antibiotic against MDR isolates of A.baumannii.