The chemical composition of the hydrodistilled essential oil of Origanum glandulosum Desf., collected from Zighoud-Youcef (Eastern Algerian), was analyzed by GC and GC/MS. 18 Components representing 93.65% % of the essential oil were detected with thymol (70.59%), tert-butylbenzene (5.76%), carvacrol (5.44%) and g-terpinene (5.04%) as the major components. The antioxidant activity of the essential oil was investigated by the use of β- carotene bleaching method.