Wollastonite clusters doped with/without CuO (0.0-10.0 g/100 g nominal CaSiO3) were prepared via precipitation route. This method is suitable for the development of wollastonite clusters, as ingenious compared with other costly methods. Wollastonite was developed after heat-treatment at 550°C/2 h, as rounded to sub rounded nano-crystals at the size of 20-50 nanometers. The cluster powders were quantitatively tested on Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Fungi (Aspergillus niger and Fusarium solani) using the agar diffusion technique. CuO-containing samples showed good activity against E. coli, P. aeruginosa and A. niger fungi. Gram negative bacteria Pseudomonas aeruginosa and fungi Aspergillus niger were detected to be most susceptible to the 5.0% CuO concentration with an inhibition zone diameter (IZD) between 30 mm and 22 mm, respectively. Moreover, E. coli showed an IZD of 20 mm with the 3.0% CuO. Stability test of sample 3.0% along 8 days, showed moderate resistance to B. subtilis, E. coli, A. niger and P. aeruginosa and weak stability against S. aureus and F. solani. Clusters of wollastonite with/without CuO prepared via wet precipitation route can be considered a novel antimicrobial agent for bone implantation.