The objective of this experiment is to formulate the lyophilized injection of water insoluble Levosimendan by using Cyclodextrin as solubility enhancer for Parenteral administration with better stability. Hydroxypropyl betacyclodextrin asSolubilizer, Trisodium citrate as buffer and Hydrochloric acid for pH adjustment were used with water for injection into 20 ml tubular vials. The filled vials were loaded into Lyophilizer and lyophilized them as per cycle. Different concentrations of Solubilizer and different pH concentrations of 5.0, 5.5, 6.0, 6.5, 7.0 and 7.5 were adjusted with 0.1 N Hydrochloric acid solutionswere usedto formulate thelyophilized formulation. The formulation F (3) which has been optimized, theresults of the cycle was observed to be optimised. Levosimendan was developed as lyophilized formulation for betterstability. The obtained results suggested that a stable formulation for drug Levosimendan was developed which wasComparable to reference listed product.