The strain Aspergillus sulphureus was evaluated for its ability to decolorize a textile dye, Reactive Black HFGR. The effect of physiochemical parameters (shaking vs static, pH, dye concentration and different carbon source) on the degradation of textile dye was studied in C-limited Czapek Dox broth. It was found that shaking favored to dye decolorization efficiency of fungal strain showed highest decolorization (93.04±1.86%). Effect of different concentration of Reactive Black HFGR dyes ranging from 50 to 300 mg/L had a significant effect on decolorization (69.94±1.09 - 93.04±1.86%) their maximal limits at 200 mg/L and increase in concentration of dye showed a negative effect on the decolorization percentage of the tested fungal strain. There was significant decolorization by the fungal strain with different pH ranging from 4 to 8. However, optimum pH was found to be pH 5. There was an influence of carbon source on decolorization as the fungus showed considerable variation in decolorization activity in the carbon supplemented medium. The fungal strain showed maximum activity with sucrose (93.04±1.86%) followed by glucose (85.31±0.59%) and fructose (78.71±1.44%). Therefore, Aspergillus sulphureus is an efficient strain for the decolorization of reactive textile dyes, and it might be a practical alternative in the dyeing wastewater treatment.