Ofloxacin is a synthetic fluorquinolone (fluoroquinolones) antibacterial agent that inhibits the super-coiling activity of bacterial DNA gyrase, halting DNA replication. Topical gel formulations of Ofloxacin were developed by using gel forming agent like Carbopol 934P. Chitosan, Neem, a n d Turmeric were used as wound healing modifiers in different concentrations. Glycerin was used as humectants. The gel formulations were characterized by IR study suggested that the formulations prepared are a physical mixture. The prepared gel formulations were evaluated for drug content, pH and rheological parameters like viscosity, spreadability and extrudability. The prepared gels were also evaluated for in-vitro diffusion study. The percent release of Ofloxacin from plain gel containing alone Ofloxacin was slow as compared to other gel formulations containing different wound healing modifiers. The formulation (F2) containing 1% Ofloxacin along with 2% chitosan showed maximum percent release (96.48%). The gels were also evaluated for in vivo wound healing activity. All the gel formulations showed more than 89% reduction in wound. The gel formulation (F7) containing Ofloxacin along with 1% chitosan and 1% turmeric showed 99.1% reduction in wound area after 12thday. Hence, from the overall study it was concluded that Ofloxacin gels along with wound healing modifiers would be promising in the effective management of wounds.