A simple, precise, accurate RP -HPLC method was developed for the quantitative estimation of atropine in Kankasava polyherbal branded formulations. The separation was achieved with a column RP C- 18 (250 mm X 4.6 mm X 5 micron) using mobile phase mixture of Methanol &10 mmol dihydrogen phosphate buffer (the pH -2.5 adjusted with orthophosphoric acid) in a ratio of 50:50 v/v at a flow rate of 1 ml/min, &analysis was screened with UV detector at 254 nm. The retention time for standard atropine sulphate was found to be 4.0667 minutes. Calibration curve was linear over concentration range 20-100 μg mL-1 Linearity was found to be r2 =0.998.The proposed method was validated for linearity, accuracy, precision and LOD, LOQ. The active content of atropine in Kankasava formulation was varying and also compared with label claim.