A simple, selective, precise and accurate High Performance liquid Chromatographic method for the analysis of Glufosinate in its formulations was developed and validated in the present study. The mobile phase consist a mixture of 5 ml/L ammonium acetate aqueous solution (containing 0.2% (v/v) formic acid) and acetonitrile in the proportion 50: 50 (v/v). This was found to give sharp peak of Glufosinate at a run time of 15 min. HPLC analysis of Glufosinate was carried out at a wave length of 195 nm with a flow rate of 1.3mL/ min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 50% to 150%. The linear regression equation was y =3650.1 x -217.1 (y = mx+c). The developed method was employed with a high degree of precision and accuracy for the analysis of Glufosinate. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The Precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is useful for the quantification of Glufosinate.