GET THE APP

Development and Validation of UV Visible Spectrophotometric Method for Estimation of Gallic acid in Tagetes Erecta Extract | Abstract
Scholars Research Library

Scholars Research Library

A-Z Journals

+44 7389645282

Der Pharmacia Lettre

Abstract

Development and Validation of UV Visible Spectrophotometric Method for Estimation of Gallic acid in Tagetes Erecta Extract

Author(s): Rajashri B. Sumbe, Ashwini R. Gawade, Albhar Shubhangi, Barwal Surekha

A rapid simple and precise UV visible spectrophotometric method has been developed for the determination of Gallic acid in methanolic extract of Tageteserecta. The spectrophotometric detection was carried out at an absorption maximum of 273 nm using ethanol as solvent. The method was validated using parameters as linearity, accuracy and precision, limit of detection and limit of quantitation as per ICH guidelines. The concentration of Gallic acid present in Tageteserecta extract was found to be 3.2 ± 0.036 w/w. The samples were prepared in ethanol and methods obeyed the beer Lambert law in concentration ranges employed for evaluation. The result of analysis has been validated statistically; hence the proposed method can be used for the reliable quantification of active marker compounds in crude drugs and its herbal formulation.