Tomato is one of the widely grown important vegetablecropandit is the second most consumed vegetable after potato in the world. Research on natural and induced mutants is primary resource to understand the genes involved in the plant development and complex physiological pathways. Chemical mutagen Ethyl Methane Sulphonate(EMS) is an effective and widely used chemical mutagen to induce point mutations at higher frequency at random locations in the genome. To attain maximum mutation density in the genome with an acceptable survival rate, standardization of optimal dose is essential. To achieve this kill curve analysis has been conducted with different EMS doses, ranging from 20 mM to 240 mM.Based on the seed germination and survival rate of the seedlings,we developed tomato mutant population of about 2,208M2 families using 80 mMEMS concentration with 46.8% germination percentage.We identified a total of 240 (10.88 %) M2 plants with morphological and developmental altered phenotypesin comparison to controlbased on primary screening andthey were categorized according to 15 primary categories.