ABO and Rhesus blood groups are the most important blood groups that are useful for transfusion purposes. The ABO and Rhesus blood groups vary in different populatios. Therefore, this study was aimed at providing information on the distribution of ABO and Rhesus blood groups among Medical students in Bowen University, Iwo, Nigeria. A total of 95 medical students were randomly selected among the medical students of Bowen University, Iwo and tested. The blood samples were collected by cubital venipuncture. A drop of each anti-sera A, B and D was added and mixed with each blood sample and then rocked gently for 60 seconds to observed agglutination. The results showed variation in the percentage frequency distribution among the students. Blood group O among the ABO blood groups has the highest percentage frequency (51.6%) while blood group AB has the least percentage frequency (3.2%). The Rhesus distribution also varies among the four ABO blood groups. The total percentage of Rhesus-positive was 93.7% and that of Rhesus-negative was found to be 6.3%. Therefore, blood group O and Rhesus-positive has the highest frequency while blood group AB and Rhesus-negative has the least frequency among the Bowen University, Iwo medical students. The present study gives vital information regarding the management of blood bank and transfusion services in the community.