Atorvastatin Calcium is a synthetic lipid-lowering agent and that belongs to the drug class known as Statins. Atorvastatin selectively and competitively inhibits the hepatic enzyme HMG-CoA reductase Nitric oxide (NO) is an important chemical messenger of many physiological and biochemical processes, including thrombosis, inflammation, immunity, vasodilation, and neurotransmission. Hyperlipidemia is a disorder of lipoprotein metabolism, which allows a number of abnormalities such as hypercholesterolemia and hypertriglyceridemia. In recent times the World Health Organization (WHO) has confirmed that by 2020, 60% of cardiovascular cases will be of Indian origin. Statins are the drug of choice in the treatment for lowering lipid levels. Statins have to turn into the leading prescription drug. Indications of statins have been really absolute over the last 5 years successive to the publication of many multicenter prospective trials. Nitric oxide can be easily detected by treating the serum with Griess reagent. Nitric oxide concentrations were determined once in a week for a period of four weeks and observed for change in the concentration. The linear regression equation was Optical Density (O.D)=0.0003 × Concentration-0.0019, (r2=0.999). These results were compared with blank plasma samples withdrawn from rats. The increased concentration was observed in the treatment group and maintained for the remaining weeks. This ensured that NO release in the blood with the administration of atorvastatin calcium is deemed to be involved in vasodilation of blood vessels and preventing the accumulation of atherosclerotic plaques and helpful maintenance of circulation.