To evaluate the effect of chemical, manure and mixed fertilizers on quantitative and morphological traits of German chamomile (Matricaria chamomile), an experiment was carried out at the field experiment of Hariri scientific foundation, Babol, iran in two seasons. A factorial experiment based on randomized complete blocks design (RCBD) with three replications was followed in the study. Results represented that quantitative and morphological traits was significantly affected by different fertilizer treatments and ecotypes. Utilization of mixed fertilizer, manure and chemical fertilizers in spring planting increased 64.27%, 47.84% and 38.33% on plant height in compare with control. Maximum of plant height and amount of essence was obtained by Isfahan ecotype. in spring planting and 50% flowering stage, utilization of manure and chemical fertilizer has similar effects on flower dry weight and application of mixed, manure and chemical fertilizers increased 325%, 189% and 104.6% on flower dry weight in compare with control. in fall and spring planting , the highest and lowest biological yield was obtained by application of mixed fertilizer and non-application of fertilizer treatments and there wasn’t significant difference between utilization of chemical and manure fertilizers on biological yield. In spring planting, utilization of mixed fertilizer, manure and chemical fertilizers increased 18.99%, 18, 20% and 10.35% on harvest index in compare with control. In fall planting, utilization of mixed fertilizer, manure and chemical fertilizers increased 25.85%, 18.26% and 13.47% on harvest index in compare with control.