Inthisresearch the effect of dam waters which are taken from four different dams around Canakkale-Turkey were used for planting Allium cepa roots and Tradescantia pallida plant species. For comparing the effects of different water sources on these plants, Allium cepaL. root tip test, and Tradescantiapallida stamen-hair mutation (Trad-SHM) test were realized respectively 72 and 96 hours after dam water application. All experiments were carried out under controlled conditions in the laboratory. According to our research results, it seems that different water resources coming from different dams has been decreased the mitotic index values in A. cepa between %8.5-%14.5 72 hours after dam water application when compared with the control group. For the T. pallida pink mutation in stamen hair cells has been increased the pink mutation events (PMEs) between 2.58-5.30 folds 96 hours after dam water application when compared with the control group. These results consistently showed that mutation levels showed differences depending on the dam locations in both plants.