The Aerva lanata belongs to the family Amaranthaceous and has the high medicinal values. The present study focused on in vitro regeneration of plant through tissue culture techniques and induction of hairy roots from stem internode explants by using various Agrobacterium rhizogenes strains such as A4, A4T and A4RS. Multiple shoots were induced from the stem explants on the MS medium containing BAP 0.5 mg/l and IAA 0.25 mg/l. Around 5.42 ±2.11 shoots were observed per explant with average shoot length of 2.19 ±1.2 cm. After 3-4 weeks of incubation the cultures were transferred to root induction medium containing 0.5 to 1.0 mg/l of IBA. Root initiation was occurred in 7-10 days on a half strength MS medium supplemented with 0.5 mg/l IBA. Healthy plants were transferred to greenhouse, were infected with bacterial strains A4, A4T and A4RS for hairy root formation from internodes.