Estimation of almotriptan malate in oral film dosage form by RP-HPLC | Abstract
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Estimation of almotriptan malate in oral film dosage form by RP-HPLC

Author(s): Pavan Kumar Kothapuvari , Swati Rawat and Darna Bhikshapathi

Almotriptan malate used to treat severe migraine headaches. Almotriptan malate oral films were prepared by solvent casting method. It is available in market as conventional tablets (Axert). A simple, precise, rapid and accurate RP- HPLC method was developed for the percentage drug release estimation of Almotriptan malate in oral film dosage form. An Intertsil C8-3, (250 x 4.6 with 5 microns particle size) and the mobile phase, consisting of 1- octane sulfonic acid sodium salt monohydrate in water adjusting the pH-3.0 with O-Phosphoric Acid: Acetonitrile in ratio of 90:10 v/v & Acetonitrile HPLC Grade: Buffer (70:30 v/v) was used as diluent in the gradient mode. The flow rate was 1.2 ml/min and the effluents were monitored at 228 nm. The retention time was 4.8 minutes for Almotriptan malate. The detector response was linear in the concentration of 2.05 -25.70 μg/mL for Almotriptan malate. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine estimation of percentage drug release in oral film dosage form of Almotriptan malate.