The present study was aimed to evaluate antioxidant property of entire herb of Euphorbia hirta by using Hydrogen peroxide radical scavenging method using ascorbic acid as standard. The ability of plant extracts to scavenge hydrogen peroxide is determined according to them. Hydrogen peroxide (H2O2) enters the human body through inhalation of vapours or mist and through eye or skin contact. In the body, H2O2 is rapidly decomposed into oxygen and water and this may produce hydroxyl radicals (OH-) that can initiate lipid per oxidation and cause DNA damage. Absorbance of hydrogen peroxide at 230nm was measured. The Euphorbia hirta can be regarded as promising entrant for natural plant sources of antioxidants with high value.