The aim of the current study was to evaluate the wound healing effect of hydroalcoholic leaf extract of Stachytarpheta jamaicensis (HLS) on Streptozotocin-induced diabetic rats. The wound healing activity was evaluated by two methods: Excision and Dead space models. Diabetes was induced in rats by a single i.p. Streptozotocin (70 mg/kg). In Excision model, wound of circular area approx. 500 mm2 was created on the back of each animal. Wound healing parameters like percentage wound contraction, period of epithelialization were observed by administering standard Mupirocin ointment (2% w/w) and HLS (2% and 5% w/w) topically. In Dead space wound model, polypropylene tubes were implanted subcutaneously and HLS extract was administered in two doses (200mg/kg and 400mg/kg). Granulation tissue was excised from implants and parameters like Tensile strength, Hydroxyproline, Hexosamine, DNA and Total protein content were measured. Animals treated with HLS had shown a significant increase in percentage wound closure, tensile strength, hydroxyproline, Hexosamine, DNA and Total protein content with a concomitant significant decrease in period of epithelialization and blood glucose levels when compared to untreated animals in a dose dependent manner. The HLS afforded significant wound healing potential in diabetic rats which may be due to the presence of phytochemical constituents such as flavanoids, triterpenes, sterols.