The aim of present research work was to formulate and evaluate microspheres of Propranolol Hydrochloride to achieve sustained release system using combination of algino- eudragit RS100 system by ionic-gelation technique. The prepared microspheres were evaluated for various parameters like percentage yield, particle size, flow property, entrapment efficiency, surface study, in-vitro drug release, X-Ray diffraction analysis, etc. It was found that all formulations showed improved flow behavior as compared to pure drug, it was observed that on increasing the polymer concentration of formulations the entrapment efficiency and particle size were increased. The surface morphology study by SEM indicated that microspheres were spherical with rough outer surface. There was no interaction between the drug and the polymers, as studied by FTIR study. In-vitro drug release study showed that on microsphere formulation its release was sustained and its release was affected by polymer concentration and it followed Higuchi model. Therefore, it can be concluded that Propranolol Hydrochloride loaded algino-eudragit RS100 microspheres can be formulated for sustained drug delivery of Propranolol Hydrochloride.