The present investigation was carried out to analyze the active constituents present in aerial parts (dry seeds) of Croton bonplandianum Baill (Euphorbiaceae) by GC-MS analysis. The presence of phytochemical compounds was screened by qualitative method. Totally 4 bioactive Phytochemical compounds were identified in the n-hexane extract of Croton species, the components were identified by comparing their relation indices and mass spectra. Fragmentation patterns with those stored on the MS-Computer library and also form the published literatures. The prevailing compounds were Squalene, (9Z, 12Z)-octadeca-9, 12-dienoic acid, methyl 12-oxo-octadec-9-enoate- and phytol. The research revealed the potential of hexane extract of Croton bonplandianum Baill seeds as a good source of phytochemicals that justify the use of this plant for its various ailments by traditional practitioners.