Genipin (Gn) initiated crosslinked hydrogel composed of sericin and chitosan was developed and evaluated for its wound healing potential in animal model. The prepared hydrogels were characterized for crosslinking degree, swelling studies, bulk and true density, pore volume, porosity, SEM, FTIR, MS/MS analysis, texture properties and rheological measurements. The hydrogels containing different concentration of Gn showed differences in morphology, swelling ability and physical properties. Crosslinking degree, FTIR studies and MS/MS analysis noticeably indicated the crosslinking of sericin and chitosan in the presence of Gn. The results of in-vivo studies indicated that 0.8G-S/C-H groups endorsed faster wound healing, enhanced reepithelialization and wound closure in albino rats when compared to the control and positive control groups. These results prove that Sericin/Chitosan hydrogels to be promising materials for wound healing.