Diabetic retinopathy (DR), a microvascular disorder affects majorly the retina of the eye. It is the major cause of permanent blindness in type II diabetic patients. Many genes have been identified for its pathogenesis. Endoglin (ENG) is one of the candidate gene involved in the DR. The physicochemical properties and structure of the ENG protein was studied using computational tools. ENG protein belongs to the Zona-pellucida superfamily. The structure of the protein revealed that the presence of maximum number of random coils as its secondary structure elements. The 3D structure was modelled using Swiss model workspace and the same was validated. This study gives an outlook of endoglin protein to carry out further research on preventing the pathogenesis of disease.