Our research work has been indicated cisplatin nanoparticles encompass natural polymer using natural as a polymer formulated for the enhanced anti-cancer activity. Nano precipitation method has been selected for the preparation of cisplatin comprising natural Gumghatti as polymer. Prepared nanoparticles have exposed the average particle size, polydispersity index and zeta potential of 143.4 nm, 0.16 and 28.5 mV respectively. Further the encapsulation efficiency, percentage drug loading and percentage yield for all the formulations were substantial, especially for the trial 6 the values observed were 93.56, 83.55 and 76.54 respectively. The in vitro release of cisplatin Gumghatti nano formulations and were determined by dissolution tester by USP apparatus II in 900 ml phosphate buffer pH 6.8. The dissolution media were maintained at 37°C ± 0.5°C with a paddle rotation speed at 50 rpm. The amount of drug used was equivalent to 15 mg at specified time intervals (5, 10 15, 20, 25 30 60, 90 and 120 minutes) The results shows that prepared nanoparticles having 98.53% of drug release in 120 minutes, further it indicates more than 95% of drug release in 2 hours. In vitro anticancer activity has been performed using cell lines the results indicated that the enhanced anticancer activity of the prepared nano formulation.