To evaluate the in vitro antioxidant activities and to estimate total phenol and flavonoids of the crude ethanolic extract from the bark and leaf of the plant Pleiospermium alatum. The shade dried powder ofbark and leaf was extracted with ethanol by using soxhlet extractor and crude extract was used for in vitro antioxidant activity by DPPH, nitric oxide, iron chelating, hydroxyl radical, super oxide radical scavenging, total antioxidant and FRAP assay methods. Total phenols and flavonoids also estimated. The results revealed that P. alatumethanolic extract have higher antioxidant property and contains large amount of phenols and flavonoids. This study helps to predict it is a better source of natural antioxidant and contains large amount of phenols and flavonoids which can be used as drugs and further investigation may lead to the development of drug formulation.