In the first stage of our research, C. officinalis and C. arvensis plants having medicinal and economical important were grown from embryo in the MS0 nutrient medium. In the second stage, callus culture was started with leaf explants of C. officinalis and C. arvensis plants and optimized with measuring of biomass in the MS0, MS1, MS3, MS4, MS6 nutrient mediums which were supplemented with combination of different concentrations of auxin and cytokinin. The statistical analysis relating to this measurements of callus biomass was conducted. The most favorable nutrient medium was established to be MS1 (1mg/l NAA+1mg/l BAP) for both C. officinalis and C. arvensis species during four different subculture from the 0. day. The most effective MS nutrient medium was determined to be MS1, MS4, MS3, MS6 and MS0, respectively.