The present study was performed to determine the influence of prebiotic Immunowall on growth performance, body composition and immunophysiological variables in juvenile great sturgeon, Huso huso. After a 4-week acclimatization period, a total of 270 juveniles of great sturgeon weighing 95.30 ± 8.99 g were randomly distributed into 9 fiberglass tanks and kept at a density of 30 fish per tank for a period of 8 weeks. Different levels of Immunowall including 0% (Control), 1% and 3% were tested in three replicate groups. At the end of the feeding trial, blood sampling and body composition analysis were conducted. Final weight, final length, body weight increase, specific growth rate, feed conversion ratio, protein efficiency ratio and condition factor were significantly (P<0.05) improved by Immunowall at 1% and 3% compared to the control. Body composition analysis showed significant differences among the experimental groups (P<0.05). Mean corpuscular volume, mean corpuscular hemoglobin and albumin showed significantly (P<0.05) higher levels in Immunowall-fed groups compared to the control. Immunoglobulin M concentration and lysozyme activity in fish fed Immunowall at 3% were higher than the control group. Based on obtained results, it can be declared that Immunowall can enhance growth performance and improve some immunophysiological variables in great sturgeon.